Author/Authors :
Dehbidi ، Sahar Department of Biology - Faculty of Biological Science - Nour Danesh Institute of Higher Education , Farokhizadeh ، Zhaleh Department of Biology - Faculty of Biological Science - Nour Danesh Institute of Higher Education , Karimi ، Mohammad Hossein Shiraz Transplant Research Center - Shiraz University of Medical Sciences , Afshari ، Afsoon Shiraz Transplant Research Center - Shiraz University of Medical Sciences , Behmanesh ، Mehrdad Department of Genetics - Faculty of Biological Sciences - Tarbiat Modares University , Sanati ، Mohammad Hossein Department of Medical Genetics - National Institute for Genetic Engineering and Biotechnology , Geramizadeh ، Bita Shiraz Transplant Research Center - Shiraz University of Medical Sciences , Yaghobi ، Ramin Shiraz Transplant Research Center - Shiraz University of Medical Sciences
Abstract :
Background: Genetic polymorphism in he miRN Asequence might alterm iRNAexpression and/or maturation, which is associated with the development and progression of hepatocellular carcinoma (HCC) in liver transplant patients. Objectives: Therefore, the prevalence of miRNA-146a G C (rs2910164), miRNA-499A G (rs3746444), miRNA-149C T (rs2292832), and miRNA-196a-2 C T (rs11614913) gene polymorphisms was evaluated in liver recipients with HCC with or without experiencing graft rejection. Methods: In a cross-sectional study, tissue samples were collected from 60 HCC patients who underwent liver transplant surgery at Namazi Hospital, Shiraz, Iran, in 2013 - 2015. A control group consisting of 120 individuals was randomly selected, as well. The genomic DNA was extracted from collected tissues and blood samples. The miRNA-146a (rs2910164), miRNA-499 (rs3746444), miRNA- 149 (rs2292832), and miRNA-196a-2 (rs11614913) gene polymorphisms were evaluated in patients with HCC using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: The CC genotype and C allele of the miRNA-146a (rs2910164) polymorphism were significantly associated with the increased risk of transplant rejection in patients with HCC (P = 0.05 and P = 0.05, respectively). The CC genotype and C allele of the miRNA-146a (rs2910164) were also significantly more frequent in male liver transplant patients who experienced acute rejection than in non-rejected ones (P = 0.05 and P = 0.03, respectively). However, no significant association was found between the genotypes and alleles of miRNA-499 (rs3746444), miRNA-149 (rs2292832), and miRNA-196a-2 (rs11614913) polymorphisms and HCC outcomes in liver transplant recipients. Conclusions: The importance of the CC genotype and C allele of the miRNA-146a (rs2910164) polymorphism in increasing the risk of transplant rejection was confirmed, but it needs further studies in larger populations.
Keywords :
Hepatocellular Carcinoma , MicroRNA , Liver Transplantation , Rejection , Polymorphism
