Title of article :
Infrared Free Electron Laser or Polarized Ultraviolet Photolysis of Hierarchical and Chiral Components of Interleukin-6, AlanylAlanine and Alanine
Author/Authors :
Fujisawa, Nanami Department of Chemistry - Faculty of Science - Tokyo University of Science - 1-3 Kagurazaka - Shinjuku-ku - Tokyo 162-8601 - Japan , Onami, Yuika Department of Chemistry - Faculty of Science - Tokyo University of Science - 1-3 Kagurazaka - Shinjuku-ku - Tokyo 162-8601 - Japan , Kawasaki, Takayasu FEL-TUS - Tokyo University of Science - 2641 Yamazaki - Noda - Chiba 278-8510 - Japan , Haraguchi, Tomoyuki Department of Chemistry - Faculty of Science - Tokyo University of Science - 1-3 Kagurazaka - Shinjuku-ku - Tokyo 162-8601 - Japan , Akitsu, Takashiro Department of Chemistry - Faculty of Science - Tokyo University of Science - 1-3 Kagurazaka - Shinjuku-ku - Tokyo 162-8601 - Japan , Tsukiyama, Koichi Department of Chemistry - Faculty of Science - Tokyo University of Science - 1-3 Kagurazaka - Shinjuku-ku - Tokyo 162-8601 - Japan
Abstract :
Interleukin-6 (IL-6) could be decomposed by irradiation of IR-FEL (Infrared free electron laser). Using circularly polarized and other UV light and IR-FEL light, photolysis of hierarchical components of cast films of IL-6, namely deuterated aqueous solutions of enantiomers of dipeptide (L-alanyl-L-alanine (Ala-ala) or D-alanyl-D-alanine) and enantiomers of amino acid (L-alanine (Ala) or D-alanine) was investigated whether specific bonds can be broken by absorption of light (not due to heat). In addition, IR-FEL irradiation to powder as well as crystal structure determination for L-Ala and D-Ala at 173 and 293 K were also carried out to confirm reproducibility in the solid state about long-lasting controversy about Salam’ hypothesis associated with chirality exhibiting structural phase transition at different temperature. Subunits of IL-6 (dipeptide and amino acid) could not be decomposed by polarized IR-FEL nor UV (ultraviolet) light regardless of their chirality. All experimental methods tested in this study failed to prove Salam's hypothesis, positively. Consequently, secondary structure of IL-6 was found to be easier to be damaged by IR-FEL than covalent bonds.
Keywords :
Interleukin 6 , alanyl-alanine , alanine , IR-FEL , Polarized light , Salam hypothesis
Journal title :
Trends in Peptide and Protein Sciences
