Author/Authors :
Jahanbani, S.S.A. Department of Anatomical Sciences - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Rezaeyan, A.H. Department of Medical Physics - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Ghaffari, H. Department of Medical Physics - School of Medicine - Iran University of Medical Sciences, Tehran, Iran , Babae, A.R. Department of Anatomical Sciences - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Haddadi, G.H. Department of Radiology - School of Paramedical Sciences - Shiraz University of Medical Sciences, Shiraz, Iran , Moeni, M.M. Pathology and Stem Cell Research Center - Department of Pathology - Afzalipour Hospital - Kerman University of Medical Sciences, Kerman, Ira , Jomehzadeh, A. Department of Medical Physics - Kerman University of Medical Sciences, Kerman, Iran , Moatamedi, S. Department of physiology and pharmacology - Faculty of Medicine - Kerman University of Medical Sciences, Kerman, Iran , Eftekhar-Vaghefi, S.H. Department of Anatomical Sciences - Afzalipour School of Medicine - Kerman University of Medical Sciences, Kerman, Iran
Abstract :
Background: Radiation-induced lung injury is a common complication after esophageal, breast, etc. cancer radiotherapy. We aimed to evaluate the radioprotective effect of arbutin on acute radiation-induced lung damage in male rats. Materials and Methods: Fifty-two male Wistar rats were divided into 4 groups: (i) control group (n = 10), (ii) vehicle group (n = 10, received distilled water intraperitoneally (ip)), (iii) X-irradiation only (n=16, chest was irradiated to a single dose of 20 Gy x-rays) and (iv) arbutin + X-irradiation (n=16, 75 mg/kg of arbutin 2 hours before irradiation (ip), and then their chest was exposed to 20 Gy x-rays). For histopathological investigation, 8 animals of each group were sacrificed 8 weeks after treatment and the rest of them were sacrificed 3 months after treatment. Results: The histopathological analysis in 8 weeks after X-irradiation showed that there was a significant increase in inflammatory in X-irradiation only group compared to control group. The administration of arbutin 2 hours prior to X-irradiation significantly reduced inflammation and inflammatory factors such as macrophages, mast cell and neutrophil in arbutin + X-irradiation group compared to X-irradiation only group (P<0.05). The histopathologic investigation performed 3 months after lung irradiation indicated a significant reduction in fibrosis formation in arbutin + X-irradiation group compared to irradiation only group (P<0.05). Localized chest X-irradiation with 20 Gy caused histopathologic damage to the lungs for short -term. Conclusion: Arbutin has a great potential in reducing the histopathologic damage to lung tissue after thoracic irradiation.
Keywords :
Arbutin , lung irradiation , radioprotector , pneumonia , fibrosis
