Author/Authors :
Liu, Binjian 161st Central Hospital of P.L.A. - Laboratory department, China , Lü, Xin Fourth Military Medical University - Department of Microbiology, China , Qi, Chaoling 161st Central Hospital of P.L.A. - Laboratory department, China , Zheng, Shuhui 161st Central Hospital of P.L.A. - Laboratory department, China , Zhou, Muxiu 161st Central Hospital of P.L.A. - Laboratory department, China , Wang, Jianmin Third Military Medical University - Institute of Surgery Research, China , Yin, Wen Fourth Military Medical University - Department of Microbiology, China
Abstract :
Background: Binding of keratinocyte growth factor (KGF) to the KGF receptor (KGFR) plays an important role in the recovery of alveolar epithelial cells from acute lung injury (ALI). Objectives: To evaluate the effect of gene therapy via adenovirus gene transfer of KGFR on the treatment of ALI. Methods: Sprague–Dawley rats were divided into four groups: normal controls, injury controls, normal adenovirus transduced group and injury adenovirus transduced group. The ALI model was induced by lipopolysaccharide (LPS) injection. Recombinant adenovirus (AdEasy-KGFR) was injected via the tail vein. Expression of the sodium (Na^+) channel in rat alveolar type II (ATII) epithelial cells was determined by PCR, immunohistochemistry and immunoelectron microscopy of rat lung tissues.Results: Gene expression of the Na^+ channel and KGFR in ATII cells was higher in the normal adenovirus transduced group than the three other groups; expression of these two genes in the injury adenovirus transduced group was higher than the injury control group. Na^+ channel protein expression was lower in the injury adenovirus transduced group but higher than the injury control group. Conclusions: KGFR over-expression induced Na channel expression could potentially be beneficial for ALI therapy.
Keywords :
Keratinocyte growth factor , receptor (KGFR) , sodium (Na+) channel , rat model , acute lung injury (ALI) , gene therapy
