Author/Authors :
Elom, Aglago Kouassivi Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Imane, El Menchawy Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Kaoutar, Benjeddou Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Khalid, El Kari Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Asmaa, El Hamdouchi Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Mehdi, Azlaf Ministry of Education - School Health Service, Morocco , Mehdi, Azlaf Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Noureddine, El Haloui Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , Hassan, Aguenaou Ibn Tofail University-CNESTEN - URAC 39 - Joint Unit of Research in Nutrition and Food Sciences, Morocco , elom, a.k. joint unit of research in nutrition and food sciences,urac 39, Morocco , imane, e.m. joint unit of research in nutrition and food sciences,urac 39, Morocco , kaoutar, b. joint unit of research in nutrition and food sciences,urac 39, Morocco , khalid, e.k. joint unit of research in nutrition and food sciences,urac 39, Morocco , asmaa, e.h. joint unit of research in nutrition and food sciences,urac 39, Morocco , mehdi, a. joint unit of research in nutrition and food sciences,urac 39,ibn tofail university-cnesten,morocco,school health service, Morocco , noureddine, e.h. joint unit of research in nutrition and food sciences,urac 39, Morocco , hassan, a. joint unit of research in nutrition and food sciences,urac 39, Morocco
Abstract :
Background: Although high-performance liquid chromatography (HPLC) is the commonly used method for the analysis of retinol in biological samples, simple and rapid test kits are available. Objectives: This study compared a rapid test kit (ICHECK Fluoro®) to HPLC for the assessment of serum retinol concentrations. Methods: For the analysis by HPLC, sample preparation included standard deproteinization and extraction phases. The analysis by ICHECK was performed by injecting serum into IEX reagent vials (n=89) and mixing manually for separation. After precipitation of the proteins, the vial was introduced into the chamber of the ICHECK Fluoro and analysed at 0 min (ICHECK0min) and 15 min later (ICHECK15min). Bland and Altman approach was applied to test the agreement between HPLC and ICHECK. Results: Mean HPLC, ICHECK0min and ICHECK15min values were 421.2±106.0 μg/L, 423.1±118.3 μg/L and 413.2±107.6 μg/L, respectively. Retinol concentrations significantly decreased in the IEX solution over time (p 0.001). No significant proportional bias was observed between HPLC and ICHECK0min (r-0.038, p=0.73) and ICHECK15min (r=-0.024, p=0.82). Fixed biases (HPLC minus ICHECK) for ICHECK0min and ICHECK15min were respectively -1.9±23.1 μg/l (p=0.45) and 8.0±22.7 μg/l (p=0.002). Conclusion: ICHECK Fluoro may offer a reliable mean for assessing serum retinol for measurements performed with no significant time delay.
