Synergetic effects of Chick Embryo Extract and Gold Nanoparticles for Matching Skin Flap Regeneration

Background and objective: In the present paper, impacts of gold Nanoparticles (AUNPs) and Chick Embryo Extract Therapy (CEET) were investigated on the wound healing promoting properties that match the dynamic healing process of skin flap regeneration. Methods: AUNPs were developed using the high-intensity short-pulse beams mode of neodymium-doped: Yttrium, Aluminum, and Garnet photoablation method. Also, 45-μg extract from a 4-6 day chick embryo was used as a protein source for CEET. Dorsal Random Skin Flaps (RSFs) were created in forty-five Albino Wistar rats and they were accidentally assigned to 3 groups; G(C); group did not receive any treatment (control), G (AUNP); treated only with AUNPs, and G(CE+AUNP) the rats treated with AUNPs and CEET. During a random skin flap animal model, AuNPs and CE were used as solutions and convenient to deliver into the skin flap. The skin flap survival rate was evaluated in the following by a camcorder, and tissue studies were done at days 3, 6, and 9 after surgery by the hematoxylin eosin and Masson trichrome staining. Result: Rats treated with the AUNPs and CEET exhibited remarkably increased flap survival than the others. Tissue studies showed that AUNPs and CEET caused induction of forming the new blood vessels and stimulated the inflammationcausing reaction in an initial phase of wound healing. Conclusion: Using AUNPs with CEET increases the potential for a dynamic recovery of skin flap regeneration.