Author/Authors :
Cano, A Diaz Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico , Sandoval, S. Jiménez Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico , Vorobiev, Y. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico , Melgarejo, F. Rodriguez Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico , Torchynska, T. V. Instituto Politécnico Nacional (IPN) - Escuela Superior de Física y Matemáticas (ESFM), Mexico
Abstract :
The article presents the results of analysis of Raman scattering spectra of non-conjugated and bioconjugated CdSe/ZnS core-shell quantum dots (QDs). Commercial CdSe/ZnS QDs used covered by polymer are characterized by color emission with the maxima at 605-610 nm (2.03-2.05 eV). The bioconjugation process is performed to biomolecules-the antihuman Interleukin 10 (IL10) antibodies (mab). Raman scattering spectra measured at room temperature with excitation by a He-Ne laser line (632.8 nm) demonstrate two groups of peaks: (1) related to the Si substrate at 230-460,522,610,670,940-1040 cm(-1) and (2) to the PEG polymer on the QD surface in the range of 837-3320 cm(-1). It is revealed that the CdSe/ZnS QD bioconjugation to the antihuman Interleukin 10 antibodies is accompanied with the dramatic changes in the intensity of the Raman lines of both types: the intensity of the Si related line increases six- or ten-fold,but the intensity of the polymer related line decreases ten-fold. The models explaining the mentioned effects in Raman scattering spectra have been discussed.
