POLYMORPHISMS OF CYP1B1 AND COMTGENES AND THE RISK OF ENDOMETRIAL CANCER IN SOUTH IRAQI WOMEN

This study was designed to study the polymorphisms of CYP1B1 and COMT genes and the risk of endometrial cancer in south Iraqi women. A total of fifty patient with endometrial cancer, aged between 15-72 year were contacted after surgery in cancer centre in Basrah, and fifty healthy women with same age and state were served as control. The blood samples were collected in sterilized tube with EDTA for DNA extraction and PCR experiments. The results showed that the frequency of CYP1B1 homozygous wild type Leu /Leu was 14% and 68% in patients and control respectively, wile the homozygous mutant Val /Val was 32% and 28%, respectively. The heterozygous mutant allele Leu /Val was 54% in endometrial cancer cases compared to 4% in control. The statistical analysis showed increased risk of endometrial cancer with homozygous mutant Val/Val genotype with an OR of 5.55 (95% CI 2.578- 11.951 ) and with heterozygous Leu/Val with an OR of 65.5 (59% CI = 20.407-210.687 ). When the patients stratified according to different stages , we found no association between the endometrial caner and stage IC, the risk increased to two fold with stage IIB , while in stage IIIA and IVB the risk was highly increased to more than six fold with an OR of 6.138 and 6.277, respectively . The frequency of COMT homozygous wild type Met/Met genotype was 2% in patients and 70% in controls, while the homozygous mutant Val/Val genotype was 40% and 14% in cases and controls respectively. The heterozygous allele Val/Met increased to 58% in Cassese compared to 16% in controls. When stratified the patients according to different stages, the risk of cancer increased to 2 folds with stage IC with an OR of 2.66 ( 95% CI = 0.686-10.356 )and to 4 folds with stage IIB (OR of 4.0, 95% CI = 0.849- 18.836), while the risk was less than 2 with stage IIIA and IVB with an OR of 1.777 and 1.333 respectively. According to the grade of the cancer, the OR was 1.714 (95% CI=0.468-6.499) with grade II. The risk highly increased with grade III with an OR of 6.584 (CI=6.210-16.25). The risk of endometrial cancer increased to 2.5 fold in patient how had CYP1B1 mutant allele Leu/Val and COMT mutant allele.