Title of article
EXTRACTION AND PURIFICATION OF MANNOSE-SPECIFIC LECTIN FROM
Author/Authors
AL-Thwaini, Amina N. University of Baghdad - Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, Iraq , Al-Maeni, Safaa A. Baghdad University - Institute of Genetic Engineering and Biotechnolog, Iraq , Zeki, Sura F.S. Baghdad University - Institute of Genetic Engineering and Biotechnolog, Iraq
From page
150
To page
157
Abstract
This study included isolation and Purification of Mannose-specific lectin (type 1 pili) from E. coli strain isolated from a human urinary tract infection. The pili were removed from the bacteria by mechanical shearing, precipitated out of solution by ammonium sulfate at a final concentration of (20%). The purification of the pili was carried out by ion exchange chromatography. There was one identical peak correspondence with the hemagglutination activity of the lectin with guinea pig erythrocytes. The further purification procedure was achieved by using gel filtration on Sepharose 6B column.
Keywords
Mannose , specific lectin , Type 1 pili , E. coli , Hemagglutination activity.
Journal title
iraqi journal of biotechnology
Journal title
iraqi journal of biotechnology
Record number
2636956
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