USE OF TEETH SAMPLES FOR GENDERDETERMINATION BY PCR IN IRAQI CASE

Identification by DNA analysis has proven valuable when the use of traditional forensic identification methods such as fingerprints or dental radiographs is difficult or impossible in situations such as explosions. Identification is even more difficult because human remains are often fragmented and may be commingled. Teeth are a useful source of DNA and can often survive extreme environmental conditions. DNA were extracted from 10 male and 10 female teeth samples ranging in age from 24 to 47 year subjects for dental treatments were used as material that contains the Amelogenin gene(amel), which is the key of sex determination in our study .PCR reaction was performed for a series of dilutions prepared from 10ng DNA to assess the minimal amount of DNA required for PCR amplification which is the primary concentration of the extracted DNA from dental materials of all 20 samples. The PCR products of the 1:100 DNA dilutions which is consider the optimum concentration. The polyacrylamide gel showed two bands with molecular weight (218bp and 212bp) for male samples and one band with molecular weight (212bp) for female samples. The results shown that the amel gene serves as a good marker for sex determination in the Iraqi population and the PCR-based method was sensitive and proved to be successful for sex determination with a complete specificity.