EVALUATION OF IMMUNOGLOBULIN M (IGM) AND INTERLEUKIN 2 (IL-2) ASSAY IN TRUE INFECTION OF ENTAMOEBA HISTOLYTICA

Eighty six sera samples were collected from patient suffering from gastrointestinal symptoms (less than one year-67 years old) had been established from the beginning of May to the end of November 2010 who attends to the Maternity and Childhood Teaching Hospital and Al-Diwania Education Hospital in Al-Diwania Governorate. Nested polymerase reaction and restriction endouclase were done previously and classified the specimen into positive for Entamoeba histolytica mixed with E. dispar , positive for E. dispar only and negative for Entamoeba sp. and usually control groups. IL-2 was detected by using ELISA in microtiter plate and designed the concentration of it in the serum. Data were translated into a computerized database structure. An expert statistical advice was sought for. Statistical analyses were computer assisted using SPSS version 13. The result showed there was an elevation in the median concentration of IgM and IL-2 in positive group for E. histolytica mixed with E. dispar in comparing with control groups, and those which was negative for Enatmoeba and positive for E.dispre only. In conclusion the levels of the serum. IgM and IL-2 which are a mediator of inflammation gave a high sensitivity and specificity in relationship with invasive amoebasis due to the high titter of IgM and IL-2, so it can be used in early diagnosis of E. histolytica infection.