Title of article
DETECTION OF A, A- MUTATIONS OF G6PD GENE ON MOLECULAR LEVEL IN IRAQI POPULATION
Author/Authors
Al-Temmemy, Rana A. University of Baghdad - Institute of Genetic Engineering and Biotechnology for postgraduate studies, Iraq , Al-Jumaily, Essam F. Baghdad University - Institute of Genetic Engineering and Biotechnology for postgraduate studies, Iraq , Al-Murran, Waleed i The University of Plymouth - School of Biological Sciences, iRAQ , Aleyasin, Ahmad National Institute of Genetic Engineering and Biotechnology, ايران
From page
357
To page
368
Abstract
The study involved the extraction of deoxyribonucleic acid (DNA) from 71 samples of random G6PD patients and 85 samples apparently healthy individuals from different Iraqi populations respectively, which was then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzymes (Nla III and Fok I) to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely A and A-. The results of the current study showed that Iraqis were affected by G6PD deficiency in a percentage 7.2%and showed that the affected cases were attributed to A mutation in 4.2%while 0%was recorded for A- mutation.
Keywords
G6pd Detection , Mutations , Rflp
Journal title
iraqi journal of biotechnology
Journal title
iraqi journal of biotechnology
Record number
2637074
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