Purification of Exotoxin A from Locally Isolate of Pseudomonas aeruginosa

Twenty-five isolates of Pseudomonas aeruginosa were isolated from wounds, burns, inflammation of the urinary tract(UTI), sputum and ear infections. These isolates were found producing exotoxin A as indicated by ELISA test. Isolates from different infections (burn, wounds and UTI) were selected according to their productivity. An amount of 0.045, 0.064 and 0.040 mg/ml protein which represent and 23.31, 29.73 and 19.01 ng/ml exotoxin A were produced respectively. Extoxoxin A was partially purified using precipitation by 90% ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B was using to detection the molecular weight for the purified toxin was estimated as 65 KD.