Title of article
Molecular Study of Azithromycin-Resistant P. aeruginosa
Author/Authors
al-rubaye, dalal s. university of baghdad - college of science - department of biotechnology, Iraq , al-bassam, wasan w. university of baghdad - college of science - department of biotechnology, Iraq , hamod, mohammed ab. l. university of baghdad - college of science - dept. of biotechnology, Iraq , al-rubaye, ilham ab. h. k. ministry of industry and minerals - al-razi center for research and diagnostic kits, Iraq
From page
71
To page
79
Abstract
The aim of the present study was to investigate the occurrence of azithromycin resistance among P. aeruginosa swabs isolates. Out of 60 pus, burn and wound swabs, 16 (26.6%) P. aeruginosa were isolated. The susceptibilities of the P. aeruginosa isolates to the macrolides azithromycin, chloramphenicol and aminoglycosides streptomycin, gentamicin and kanamycin were evaluated by disc diffusion experiments. The azithromycin was observed to be less resistance with high susceptibilities rate 13 (81.25) as compared to aminoglycosides and chloramphenicol. Partial 23S rDNA gene sequences of 12 isolates demonstrate new single base substitution in the resistant isolates in positions A1807G, C1808A, A1823G and A1819G which confer the azithromycin resistance with low frequency rate 0.9 for each one. As we recommend the azithromycin is the drug of choice in P. aeruginosa treatment, further study is needed to whole 23S rDNA gene in local isolates to identify mutations outside the partial selected region.
Keywords
Azithromycin , 23Srrna , P. aeruginosa
Journal title
iraqi journal of biotechnology
Journal title
iraqi journal of biotechnology
Record number
2637486
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