Author/Authors :
Salo, Wisam H. University of Baghdad - Institute of genetic engineering biotechnology for post graduate studies, Iraq , Imad, Ali University of Baghdad - Institute of genetic engineering biotechnology for post graduate studies, Iraq , Nasir, Gulboy A. University of Baghdad - College of Agriculture - Division of Basic sciences, Iraq
Abstract :
The degree of genetic divergence was estimated in six Iraqi soft wheat genotypes, four exotic genotypes, one produced by hybridization and one produced by gamma irradiation, through random amplified polymorphic DNA methodology. A total of 42 DNA fragments were generated by the 3 random primers, with an average of about 7.4 bands per primer. The 42 fragments showed polymorphism among the six wheat genotypes. Jaccard similarity matrix ranged from 25 to 66.7%, which indicated a high genetic diversity among the genotypes. We conclude that random amplified polymorphic DNA analysis can be used for the characterization and grouping of wheat genotypes; these results will be helpful in our wheat breeding program.
