Optimum Condition of Inulinase Production from Bacillus cereus (Be9)

Fifty isolates of Bacillus spp were obtained from rhizosphere soil of compositae plant roots. The ability of inulinase production by these isolates was screened. Bacillus Be9, which isolated from soil of lettuce root, was the highest inulinase producer; it was identified as Bacillus cereus. Optimal culture medium and condition for inulinase production were determinatd; the highest inulinase production was obtained when the bacteria was cultured in inulin medium which contained 0.5% inulin, 0.4% peptone as carbon and nitrogen source at pH 7.0 inoculated with 1ml of bacterial suspension and incubated at 40˚C for 48hrs.