The use of Spectrophotometric Batch and Flow injection Estimation of Clonazepam drug in Pure and Pharmaceutical Preparations

Two simple, sensitive, accurate, and rapid spectrophotometric batch and FIA methods have been developed for the estimation of clonazepam in pure and in a commercial tablet pharmaceutical preparations. They are based on the oxidative coupling reaction of clonazepam (CZP) with Resorcinol in the presence of sodium periodate exhibiting red coloured products obtained with a maximum absorbance at (𝜆max) at 510 nm .The reaction conditions (chemical and physical parameters) were studied and optimized. The produced coloured oxidative coupling reaction was stable for more than 2 h. The linear range for the batch and FI methods determination of CZP were 3-40, 25-250 μg mL^-1 and the detection limit, were1.26, 13.87μg mL^-1 for both methods respectively. A statistical comparison of these results with those obtained by the British pharmacopoeia (BP) procedure using the Student t-test and variance ratio F-test shows a good agreement and indicates no significant difference in accuracy and precision at the 95% confidence..