Production, Purification and Characterization of Uricase Produced by Pseudomonas aeruginosa

In this study, detection of uricase production from Pseudomonas aeruginosa isolates was done by applying colorimetric method, Uricase was purified from the most potent isolate by precipitation using ammonium sulphate (80% saturation) then purification was achieved using DEAE –Cellulose ion exchange and Sepharose 6B gel filtration chromatography column, 16.4% of total enzyme was recovered with specific activity 2337.5U/mg and 22.21folds of purification. Characterization of uricase involved detection of optimal conditions for uricase activity, the maximal activity was obtained at temperature 45ºC,while uricase appeared to be stable at 40ºC. Uricase showed optimal activity at pH 9 while pH stability was in the range of 7.5-9. Molecular weight of uricase obtained in this study determined by sodium dedosyl sulfate electrophoresis (SDS-PAGE) technique was approximately 35kDa.