Using ISSR markers to build a phylogenetic of Barley Genotypes

This study is attempts to build a phylogenetic between nine Iraqi barley genotypes based on ISSR-PCR analysis by determine the level of genetic similarity among them. Nine issr primers used in this study produced 41 bands across nine studied varieties. Of these bands, 28 bands were polymorphic and the remaining monomorphic bands were 13. The average polymorphic rate was 70.5% ranged between 25%-100% , and average of polymorphic bands /primer was 4.5.The size of the amplified bands ranged 140-1600 bp. It was generated a 5 unique bands in this study, these bands can be used as a DNA profiling of all studied genotypes. The results were showed Genetic distances ranged between (0.0854-0.9897) among barley varieties. Based on phylogenic tree, a dendrogram were constructed among studied barley varieties, cluster analysis grouped the nine varieties into two main clusters depending on their ancestors and their morphological traits. The use of cultivars from various clusters and sub clusters offer the possibility of obtaining an appropriate genetic variability in hybrid population.