Author/Authors :
al-suhail, raghad g. university of baghdad - college of science - department of biology, Iraq , ali, layla f. university of baghdad - college of science - department of biology, Iraq , aufi, iman m . ministry of health - virology department, central public health laboratory, Iraq
Abstract :
Current study aimed to investigate the Respiratory Syncytial Virus (RSV) in nasal secretion and throat swab samples of hospitalized patients with symptoms of respiratory tract infection using Immunofluorescence test. Previously these samples were tested for Respiratory Syncytial Virus infection by reverse transcriptase-PCR assay. The positive samples were tested by Immunofluorescence assay in monolayer confluent of Hep 2 cell line. The results showed that the positive samples using the RT-PCR test were positive in this test. These results reveal that Immunofluorescence test is sensitive method for detection the infection with RSV.