Antibacterial and Antioxidant Activites of Secondary Metabolites of Endophytic Fungus Stemphylium radicinum (Meier, Drechs and Eddy)

Endophytic fungi are gaining interest as sources of novel bioactive metabolites. The study was carried out to screen and isolate endophytic fungus.An endophytic fungus isolated from root of calyptous plant .The fungus was identified as Stemphylium radicinum (Meier, Drechs and Eddy)based on morphological characterization. Fungal secondary metabolites was carried out by ethyl acetate solvent.The antibacterial activity was tested against five bacterial isolates. Esherichia coli, Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumonia and Streptococcus pyogenes by using a disc diffusion technique. The inhibition zones exhibited by Fungal secondary metabolites were ranged between 22.5-35.5 mm. Minimum inhibitory concentration MIC test revealed that the extract of S. radicinum exhibited a minimal inhibition values ranging between 25.0-100 ug/ml against bacterial strains .A verification of non toxicity of the fungal secondary metabolites against human blood revealed a negative test. Active compounds of the fungal secondary metabolites involves Tannins, phenols and amino acid .The antioxidant activity was analyzed using2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays .The metabolite produced by the fungi could be an alternative source of antimicrobial against clinical pathogens.