Study of antioxidant effect of Curcuma longa L. phenolic extract, sodium selenite and vit. E on some physiological and biochemical criteria of white male rats treated with chromium picolinate

The current study was designed to determine the antioxidant effects of phenolic extract of Curcuma longa L. ,sodium selenite and vitamin E against oxidative stress induced by chromium picolinate in some physiological and biochemical criteria for the blood, some antioxidants and neurotransmitters of the brain on the adult male rats. The study was conducted in the animal house of the Faculty of Science/University of Kufa on 70 animals of adult male rats aged 2.5-3 months and the weight of 200-250 gm. The results showed no significant change (p 0.05) in the average of body weight, body organ weight and liver enzymes in the rats treated with chromium picolinate only for the two periods of the experiment as well as the same results recorded in the animals treated with chromium picolinate with vitamin E and sodium selenite and chromium picolinate with the phenolic extract of Curcuma longa for six and eight weeks compared with control group. As it has been noted a significant decrease (p 0.05) in the antioxidants include superoxide dismutase, glutathione peroxidase and significant increase (p 0.05) in the malondialdehyde in the animals treated with chromium picolinate for the two period of administration compared with the control group also a significant decrease (p 0.05) in dopamine (DA) and a significant increase (p 0.05) on serotonin (5-HT) in the animals treated with chromium picolinate only in compared with control group. Moreover, the significant increase occurred (p 0.05) in the antioxidants superoxide dismutase and glutathione peroxidase levels and significant decrease(p 0.05) in malondialdehyde level in the animals treated by chromium picolinate separately with the phenolic extract of Curcuma longa, chromium picolinate with vitamin E and sodium selenite compared with the control group and the results showed a significant increase (p 0.05) in dopamine (DA) and significant decrease (p 0.05) in the serotonin (5-HT) in the groups mentioned above for a periods of six and eight weeks compared with control group. The study conclude from that the phenolic extract of Curcuma longa, sodium selenite and vitamin E have effective antioxidants against oxidative stress induced by oral administration of chromium picolinate led to an improvement of the above criteria studied, compared with control group and the best results are recorded after treatment by the phenolic extract of Curcuma longa.