Frequency of Metallo-β-Lactamases Producing Klebsiella pnumoniae in Burn Wound Isolates

From the period 1 /3 /2016 to 30 / 8 / 2016, 210 swabs were collected from the burn patients hospitalized in different hospitals in Baghdad City: Al-Karama Teaching Hospital, Special Burn Hospital, Central Teaching Laboratories, Child protection Teaching Hospital, Imam Ali Hospital. Forty two isolates (37.5 %) showed a growth of Klebsiella pneumonia, and the remaining isolates (62.5%) were belonged to other bacteria. Identification of all isolates were carried out depending on macroscopic, microscopic characterizations, conventional biochemical tests and Api 20E system. Metallo -β lactamase (MBL) enzymes were screen by three different phenotypic methods (DDST, CMDT and MHT) in which 10μg meropenem antibiotic disk has selected to all phenotypic methods.Susceptibility test done with antibiotic disks of cefotaxime, ceftazidime, aztreonam, imipenem, meropenem, gentamicin, amikacin, ciprofloxacin, tetracycline and trimethoprim-sulfamethoxazole. The percentage of resistance isolates were presented as, Tetracycline (95.23%), Cefotaxime (85.71%), Trimethoprim- Sulfamethoxazol (83.33%), Aztreonam (71.42%), Ceftazidime (69.04%), Ciprofloxacin (59.52%), Gentamycin (26.19%), Imipenem (21.42%), Meropenem and Amikacin (19.04 %).