Detection of some virulence factors with Biochemical investigation of Listeria monocytogenes associated with human infections.

This study was aimed to detect different virulence genes that might be present in the Listeria monocytogenes. 50 clinical samples were gathered from patients with different sites like (stool, urine and blood). Though, only (22) isolates were contained L.monocytogenes using conventional and traditional techniques. Strains were isolated with the following approach 8 (36%), 6 (27%), and 8 (36%) from human intestine (stool), urine, and blood samples respectively.Virulence genes were detected by using specific PCR primers which include (iap, hly andinl A). The results indicated that four isolates of L. monocytogenes possessed (hly) gene, two from urine strains 2:6 (33%), one from stool 1:8 (13%) and one from blood 1:8 (13%). In addition to that, only three isolates of inlAgave positive amplification where1:8(13%) from stool 1:6(17) from urine and 1:8 (13%) from blood.Moreover, six strains gave positive amplification for japgene where 3:8(38%) from stool and 2:6 (25%) from urine,and one fromblood1:8(13%).