ANTIBACTERIAL ACTIVITY OF VARIOUS PHYTOCONSTITUENTS OF NEEM

Antibacterial activity of various phytoconstituents of neem was studied to rationalize its traditional use. Neem leaves extract was carried out in order to isolate various phytoconstituents including Alkaloids, Steroids, Tannins, Glycosides, Flavonoids and Saponins. Two concentrations 50 mg/ml and 75 mg/ml of each phytoconstituent of neem and neem extract were applied against three bacterial strains i.e., Staphylococcus areous, Corynebacterium bovi and E.coli by using disc diffusion method. The inhibition zones were measured in millimeter with the help of a zone reader. The data show that the inhibition zones of neem extract were greater than each of the phytoconstituents, and 75 mg/ml concentration was the more effective than 50mg/ml.