BIOREMEDIATION OF WASTEWATER CONTAINING REACTIVE DYES BY Agaricus bisporus A21: EFFECT OF SUPPLEMENTS AND REDOX MEDIATORS

This study involves the development of bioremediation process for simulated solutions of reactive dyes by Agaricus bisporus A21. Initially screening trial with four Reactive dyes, viz. NOVACRON Reactive black, NOVACRON Reactive blue, NOVACRON Reactive red and NOVACRON Reactive G. Yellow was carried out using Agaricus bisporus A21. Among four dyes tested the fungus showed better decoloration efficiency (67.7 %) with NOVACRON Reactive black as compared to other dyes during a 10th day period. The effect of various operational parameters like pH, incubation temperature, carbon nitrogen additives, surfactant as well as dye concentration was optimized for enhanced decolorization of the dye under study. The effect of various redox mediators like ABTS, veratryl alcohol, MnSO4, guaiacol, glyoxlate on enzyme activity and removal of color was also investigated. After optimization of process conditions the rate of removal was enhanced up to 84 % just within 5 h .Enzymes involved in the degradation of the dye have been investigated and it was found that MnP (820 IU/mL) was the major enzyme responsible for the dye degradation. It was shown that there was a direct correlation between the observed enzyme activity and the investigated process effectiveness. The results indicated that Agaricus bisporus A21 could be used to treat dyes containing wastewater from various industries.