cpDNA SSR POLYMORPHISM IN SECONDARY GERMPLASM OF POTATO MAINTAINED IN CZECH GENE BANK

Simple Sequence Repeat (SSR) has been employed successfully in many plant species because of high polymorphism and relatively low cost. Aim of the research was to evaluate cpDNA polymorphisms in germplasm collection of 31 accessions of Czech Gene Bank belonging to 27 Solanum species using twenty-three cpSSRs markers. In total, 94 alleles were detected, i.e. 4.09 alleles per each cpSSR locus in average. The highest polymorphism was detected in the locus NTCP9 (16 alleles) and lowest were recorded by two alleles in six of loci. No polymorphism was observed in case of SSR locus NTCP19. Mean value of observed heterozygozity (Ho) was 0.57, whereas mean of Polymorphic Information Contents (PIC) was 0.49. Less variability occurred in exons (Hex=0.41) compared to introns (Hin=0.54) and intergenic regions (Higr=0.60) of the chloroplast genome. The most important result was finding a set of four SSR markers (NTCP6, NTCP8, NTCP9 and NTCP12; mean PIC = 0.795) identifying 30 from 31 studied individuals as original haplotypes; only two S. gourlai individuals were identical. It is very helpful tool for DNA fingerprinting in collections of potato genetic resources.