A NEW SENSITIVE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF ADRENALINE IN PHARMACEUTICAL PREPARATIONS

A sensitive and simple spectrophotometric method for the estimation of adrenaline (ADH) in either pure form or in pharmaceutical preparations. The method is based on the reaction of adrenaline with vanadium (V) in acidic solution to from the colored complex which absorb at λmax = 488 nm. A graph of absorbance versus concentration shows that Beer ,s low is obeyed over concentration range of (0.5–140) µg adrenaline ml-1 with a molar absorpitivity of (2.015× 103 Lit. mol-1.cm-1), a sandell sensitivity of ( 0.09 µg.cm-2) ,LOD (0.46µg.ml-1) , Recovery % (101.16± 0.97) , Erel % (1.17± 0.97) . The mole–ratio method (1:2) approved that ADH- V (V) as a structure of the complex. The optimum conditions for full colour development are described and the proposed method was satisfactorily applied for the determination of ADH in pharmaceutical preparations. The response surface method (RSM) was applied for optimization of experimental condition and data obtained were found similar.