Title of article :
While Urine and Plasma Decorin Remain Unchanged in Prostate Cancer, Prostatic Tissue Decorin Has a Prognostic Value
Author/Authors :
Rezaie, Razie Department of Genetics and Molecular Medicine - School of Medicine - Zanjan University of Medical Sciences (ZUMS), Iran , Falakian, Zeinab Department of Genetics and Molecular Medicine - School of Medicine - Zanjan University of Medical Sciences (ZUMS), Iran , Mazloomzadeh, Saeideh Department of Epidemiology and Statistics - School of Medicine - Zanjan University of Medical Sciences (ZUMS), Iran , Ayati, Mohsen Uro-Oncology Research Center - Tehran University of Medical Sciences (TUMS), Iran , Morakabati, Arman Molecular Pathology Department of Mehr General Hospital, Iran , Teimouri Dastjerdan, Mohammad Reza Urology Department of Farabi General Hospital, Iran , Zare, Mohammad Molecular Pathology Department of 17-Shahrivar General Hospital, Iran , Moghimi, Minoosh Department of Hemathology Onchology - Zanjan University of Medical Sciences (ZUMS), Iran , Shahani, Tina Department of Genetics and Molecular Medicine - School of Medicine - Zanjan University of Medical Sciences (ZUMS), Iran , Biglari, Alireza Department of Genetics and Molecular Medicine - School of Medicine - Zanjan University of Medical Sciences (ZUMS), Iran
Pages :
7
From page :
229
To page :
235
Abstract :
Numerous studies confirmed that significant decrease in tissue decorin (DCN) expression is associated to tumor progression and metastasis in certain types of cancer including prostate cancer (PC). However, the potential prognostic value of tissue DCN in PC has not yet been investigated. Methods: A total number of 40 PC and 42 patients with benign prostatic hyperplasia (BPH) were investigated for the expression levels of DCN in their prostatic tissues using real-time quantitative polymerase chain reaction and immunohistochemical analyses. Urinary and plasma DCN levels were also measured by ELISA. Results: Despite no significant changes in the mean of urine and plasma DCN concentrations between the two study groups, tissue DCN mRNA was found to be 5.5fold lower in cancer than BPH (p = 0.0001). Similarly, the stained DCN levels appeared significantly lower in cancer patients with higher Gleason Scores (8 and 9, n = 6) than those with lower Gleason Scores (6 and 7, n = 26), with a p value of 0.049. Conclusion: Here, we report, for the first time, that urine and plasma DCN does not seem to have a diagnostic value in PC, while tissue DCN could potentially be used as a prognostic marker in PC.
Keywords :
Benign prostatic hyperplasia , ELISA , Immunohistochemistry , Proteoglycans , Real-time qPCR
Journal title :
Iranian Biomedical Journal(IBJ)
Serial Year :
2020
Record number :
2643464
Link To Document :
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