SYNTHESIS OF ADENOSINE MET-ENKAPHALIN CONJUGATE WITH EXPECTED BIOLOGICAL ACTIVITY

In an effort for designing analogue with improved analgesic activity(opioid like activity) or may posses added biological activities as antimetabolite with antineoplastic antibacterial and antiviral, etc., coupled bi natural metabolites (nucleoside peptides) are proposed in this study to synthesize 1′ -deoxy-1′ -(6-amino-9-purinyl)-2′ ,3′ -o-isopropylidene-B-D-ribfuranuronic acid amide with Tyr- Gly-Gly-Phe-Met-oH. In the hope that such combination have efficient transporting system across membrane, natural penetration inside the cell and nucleus, represent an essential criteria for specifically inducing the analagesic, antiviral, anticancer and also help for exploring other expected biological activities. The analogue was synthesized by applying the conventional solution method and the coupling between peptide and nucleoside adenosine was carried out through amide linkage.Confirmity of the synthetic procedure was achieved by applying different physico-chemical analyses including, thin layer chromatography (T.L.C) and melting point (M.P.), infrared spectroscopy (IR),elemental analysis(CHN analysis), optical rotation, amino acid analysis and NMR.