Purity check of Canola Varaities / Mutants by Gas Liquid Chromatography

Screeing of twenty-three canols varaities/mutants (grown at oilseed Research Institute, Ayub Agricultural Research institute, Faislabad, Pakistan) was carried out on the bases of their fatty acid profile to moniter their purity. Fatty acid composition was determined by GC/ FID on 20% DEGS packed glass column. All the varietes/mutants varied in the composition of palmatic, streaic, oleic, linoleic, lenolenic, ecosnoic and erucic acid significantly. Out of twenty three canola varieties /mutants only four were found pure, having erucic acid less than 2%, four showed erusis acid between 2 to 5%, two between 10 to 15%, three between 20 to 30%, seven between 35 to 50% and three variates were found having erucic acid more than 50% . The estimates of simple coefficients showed highly significants negative correlation between erucic acid and other fatty acid except linolenic acid, where non-significants correlation was observed. Multiple correlation/regression analysis indicated that oleic acid contributed up to extent of 97% towards the total variation in erucic acid.