Author/Authors :
SULTANA, NAJMA University of Karachi - Faculty of Pharmacy - Research Institute of Pharmaceutical Sciences,Department of Pharmaceutical Chemistry, Pakistan , ARAYNE, MUHAMMAD SAEED University of Karachi - Department of Chemistry, Pakistan , IFTIKHAR, BENISH University of Karachi - Faculty of Pharmacy - Research Institute ofPharmaceutical Sciences, Department of Pharmaceutical Chemistry, Pakistan , NAWAZ, MUHAMMAD University of Karachi - Department of Chemistry, Pakistan
Abstract :
A sensitive high-performance liquid chromatography method was developed for the determination of atenolol in bulk drug and pharmaceutical formulation in the presence of flurbiprofen as internal standard. Separation was carried out on a Purospher Start, C18 (5mm, 250 x 4.6 mm) column with MeOH: H2O (80: 20, % v/ v) solvent systems and pH was adjusted to 2.85 with ortho-phosphoric acid. Mobile phase was pumped with a flow rate of 1 ml/ minute with UV detection at 224 nm. Standard curves were linear over the concentration range of 0.25-30 mg / ml. The inter-day and intra-day coefficients of variation were less than 1 % at different concentrations. The method was then applied to study interaction of atenolol with essential and trace elements. The combination of hydroxy and aminogroups makes atenolol a good chelating ligand. Drug metal interaction studies were carried out at 37 oC to monitor the complexation of drug with metal ions. The order of complexation of atenolol with metals was found to be copper ferric chromium zinc ferrous cobalt nickle magnesium manganese cadmium calcium.
