Author/Authors :
SATTAR, MUHAMMAD University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , AHMED, SIBTAIN University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , AHMED, SIBTAIN University of Veterinary and Animal Sciences - Department of Physiology and Biochemistry, Pakistan , SHEIKH, MUNIR AHMAD University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , HASHMl, ABU SAEED University of Agriculture - Department of Chemistry and Biochemistry, Pakistan , HASHMl, ABU SAEED University of Veterinary and Animal Sciences - Department of Physiology and Biochemistry, Pakistan
Abstract :
Brevibacterium flavum is an industrially important microorganism that is used for the production of lysine, Fermentation conditions of B. flavum were optimized for enhanced production of lysine. Yeast sludge was fermented by B. flavum under the optimized conditions to increase the lysine content The optimum pH, temperature and incubation time for B. flavum for lysine production were 7,35 °C and 48 h respectively. Maximum lysine concentration was achieved with 1 % molasses, 4 % corn steep liquor and 25 mg % protein hydrolysate of sesame meal. Fermentation of yeast sludge with B. flavum increased the lysine content from 1.54 % to 4.78 % as shown by amino acid analysis.