Title of article :
Effect of Molecular Mutagenesis on Alcohol Dehydrogenase Production by Pseudomonas fluorescens RB30
Author/Authors :
Hadi, Noora A. University of Al-Nahrain - College of Science - Department of Biotechnology, Iraq , Al-Gelawi, Majed H. University of Baghdad - College of Science - Department of Biotechnology, Iraq , Aziz, Ghazi M. Baghdad University - College of Science - Biotechnology Department, Iraq
Abstract :
Alcohol dehydrogenases are group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketons with the reduction of NAD^+ to NADH. Locally isolated Pseudomonas fluorescens RB30 was selected depending on its efficiency in producing of alcohol dehydrogenase (ADH) enzyme. Its specific activity was 13.6 U/mg proteins after extraction by using lysozyme. As an attempt to improve ADH production, Pseudomonas fluorescens RB30 was subjected to molecular mutagenesis following conjugation with E. coli PULB113, Tet^r and Km^r harboring the conjugative plasmid RP4 that carried Mini-Mu transposon, and two mutants were due to their high activities were resulted (0.82 U/ml and 0.79 U/ml respectively).
Keywords :
Pseudomonas , alcohol dehydrogenase , molecular mutagenesis
Journal title :
Al-Nahrain Journal Of Science
Journal title :
Al-Nahrain Journal Of Science
