Title of article
Infection of Galleria mellonella larvae by Steinernema affine and production of infective juveniles
Author/Authors
rahoo, a. m. university of reading - school of agriculture, policy and development, UK , rahoo, a. m. wheat research institute, Pakistan , mukhtar, t. pir mehr ali shah arid agriculture university - department of plant pathology, Pakistan , bughio, b. a. university of sindh - department of zoology, Pakistan , gowen, s. r. university of reading - school of agriculture, policy and development, UK , rahoo, r. k. university of sindh - institute of plant sciences, Pakistan
From page
65
To page
71
Abstract
The information regarding relationship between nematode dosage and infective juveniles (IJ) production are available for Steinernema affine. Therefore, in the present study the effect of inoculation doses of S. affine and extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction trays. In Baermann extraction trays, the maximum emergence of IJ of S. affine was observed at an inoculation dose of 400 IJ followed by the dose of 50 IJ. The minimum emergence was found with an inoculation dose of 100 IJ. On the other hand, in case of White traps, the maximum emergence of IJ was recorded with doses of 100 and 50 IJ. The minimum emergence of IJ was observed with a dose of 200 IJ. The relationships between inoculation doses and emergence of IJ from the cadavers for both the methods were found to be non-significant.
Keywords
Entomopathogenic nematodes , emergence , reproduction , Steinernema affine , Galleria mellonella.
Journal title
Pakistan Journal Of Nematology
Journal title
Pakistan Journal Of Nematology
Record number
2646619
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