Author/Authors :
KEONG, YEAP SWEE Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , ALITHEEN, NOORJAHAN BANU Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , MUSTAFA, SHUHAIMI Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Microbiology, Malaysia , ABDUL AZIZ, SURAINI Universiti Putra Malaysia - Department of Bioprocess Technology, Malaysia , ABDUL RAHMAN, MASHITOH Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia , ALI, ABDUL MANAF Universiti Putra Malaysia - Faculty of Biotechnology and Biomolecular Sciences - Department of Cell and Molecular Biology, Malaysia
Abstract :
In this study, the immunomodulatory effects of zerumbone isolated from Zingiber zerumbet were investigated by evaluating the effects of this compound towards the lymphocytes proliferation (mice thymocytes, mice splenocytes and human human peripheral blood mononuclear cells, PBMC), cell cycle progression and cytokine (interleukin 2 and 12) induction. Lymphocyte proliferation assay showed that zerumbone was able to activate mice thymocytes, splenocytes and PBMC at dosage dependent pattern where the best concentration was 7.5 μg/mL. Flow cytometry analysis showed the highest population of PBMC entered into G2/M phase after treatment for 72 h with 7.5 μg/mL zerumbone. The production of human interleukin-2 and human interleukin- 12 cytokines in culture supernatant from zerumbone activated lymphocytes was prominently upregulated at 24 hour and decreased from 48 h to 72 h. The above results indicate that zerumbone can be used as immunomodulatory agent which can react toward the immune cell cytokine production in dosage dependent pattern.
Keywords :
Zingiber zerumbet , zerumbone , immunomodulation , interleukin 2
