Author/Authors :
Kassaye, Lantider Ethiopian Food, Medicine and Healthcare Administration and Control Authority - Food and Medicines Quality Control Laboratory, Pharmaceutical Chemistry Department, Ethiopia , Hymete, Ariaya Addis Ababa University - School of Pharmacy - Pharmaceutical Chemistry Department, Ethiopia , Bekhit, Adnan Ahmed Addis Ababa University - School of Pharmacy - Pharmaceutical Chemistry Department, Ethiopia , Bekhit, Adnan Ahmed Alexandria University - Faculty of Pharmacy - Pharmaceutical Chemistry Department, Egypt , Genete, Getachew Ethiopian Food, Medicine and Healthcare Administration and Control Authority - Pharmaceutical Chemistry Department, Food and Medicines Quality Control Laboratory, Ethiopia
Abstract :
A validated HPLC method for the simultaneous determination of diminazene diaceturate and phenazone has been established for the analysis of the compounds in veterinary granules for injection and in bulk powders. The compounds were separated using a Symmetry RP 18 analytical column and detected by UV absorbance at 250 nm. Linearity, accuracy as well as the intra-assay precision, inter-day precision and specificity of the method were established. The limits of detection and quantification were 3.2 and 9.7 μg mL^-1 for diminazene diaceturate and 9.57 and 28.99 μg mL^-1 for phenazone. Method had the potential to determine these drugs simultaneously from dosage forms without any interference with each other.
Keywords :
Diminazene , phenazone , simultaneous determination , HPLC method.
