Author/Authors :
Dar, Ume-Kalsoom Institute of Nuclear Medicine Oncology (INMOL) - Radiopharmacy and PET Radiochemistry Division, Pakistan , Dar, Ume-Kalsoom University of the Punjab - Institute of Chemistry, Quaid-e-Azam Campus, Pakistan , Khan, Irfan Ullah Institute of Nuclear Medicine Oncology (INMOL) - Radiopharmacy and PET Radiochemistry Division, Pakistan , Javed, Muhammad Gujranwala Institute of Nuclear Medicine (GINUM), Pakistan , Ali, Muhammad University of the Punjab - School of Biological Sciences (SBS), Quaid-e-Azam Campus, Pakistan , Hyder, Waqar Institute of Nuclear Medicine Oncology (INMOL) - Radiopharmacy and PET Radiochemistry Division, Pakistan , Murad, Sohail Gujranwala Institute of Nuclear Medicine (GINUM), Pakistan , Anwar, Jamil University of the Punjab - Institute of Chemistry, Quaid-e-Azam Campus, Pakistan
Abstract :
5-Fluorouracil is a well know drug for chemotherapy of various types of cancer. In the present study, we radiolabeled 5-fluorouracil with 99mTc for a diagnostic study of cancer. After successful labeling of the drug we performed an animal study to evaluate the potential of this radiopharmaceutical as a tumor diagnostic agent. The results showed 98.1 ± 1.2 % labeling efficacy of 5-fluorouracil with 99mTc. The in vitro stability of the radiolabeled drug at room temperature at 4 hr of post-labeling was 96.5 ± 0.4 %. The binding of the radiolabeled drug with plasma proteins was 66.6 ± 3%. Partition coefficient results showed that this drug is hydrophilic in nature. Biodistribution study in rabbit models displayed faint uptake in liver. Both kidney and bladder were prominent as excretory route of the labeled drug. Bioevaluation was performed in Swiss Webster mice having naturally developed tumor. Mice were dissected, uptake of drug in various organs was studied and results showed prominent uptake in liver and tumor. Tumor was further investigated by histopathological study.
Keywords :
5 , Fluorouracil , radiolabeling , biodistribution , tumor , diagnosis
