Preparation and evaluation of 99mTc-DMSA lyophilized kit for renal imaging

Dimercaptosuccinic acid (DMSA) has been evaluated and used with technetium 99m (99mTc) in imaging of kidneys. DMSA lyophilized kits were prepared and radiolabelled with 99mTc. Paper and thin-layer chromatography have been employed using various eluent systems for the radiochemical analysis, percentage labeling and binding capacity of 99mTc-DMSA. Female albino rabbits were used for this study. Biological data obtained after intravenous injection of radiolabelled DMSA to female albino rabbits revealed 32.42% uptake and long retention time in the kidneys. On the basis of animal biodistribution data, it is suggested that DMSA when labeled with 99mTc is useful complex for renal imaging and can be successfully applied as a diagnostic tool in nuclear medicine. Clinical biodistribution and radiation dosimetry studies are planned in future.