A comparison among three different analytical methods to test the scavenging properties of different integrators against radicalic stress

The aim of the present work was to experimentally evaluate the total antioxidant capacity of different commercially available pharmaceutical integrators based on vitamin E, astaxanthin, resveratrol and blueberry using the traditional DMPD-FeCl3 spectrophotometric method and the ORAC spectrofluorimetric method, largely discussed and described in literature and considered as reference methods. The results were compared with those ones obtained with a biosensor based on superoxide dismutase (SOD) enzyme. Precision of antioxidant capacity measures for all integrators was good, generally with a R.S.D.% ≤10% for all methods employed. An evaluation was also performed of the possible influence on the total integrator antioxidant capacity from other compounds and/or excipients present in the formulations which can interfere with the main antioxidant component of the commercial integrator.