Title of article :
Cytotoxic and antimicrobial activities of Emex spinosa (L.) Campd. extract
Author/Authors :
Donia, Abd El Raheim Mohammed Salman Bin Abdulaziz University - College of Pharmacy - Pharmacognosy Department, Saudi Arabia , Donia, Abd El Raheim Mohammed Desert Research Center - Medicinal and Aromatic Plants Department, Egypt , Soliman, Gamal Abd El Hakim Salman Bin Abdulaziz University - College of Pharmacy - Pharmacology Department, Saudi Arabia , Soliman, Gamal Abd El Hakim Cairo University - College of Veterinary Medicine - Pharmacology Department, Egypt , El Sakhawy, Mohamed Abd El Monem Salman Bin Abdulaziz University - College of Medical and Applied Sciences, Saudi Arabia , Yusufoglu, Hasan Salman Bin Abdulaziz University - College of Pharmacy - Pharmacognosy Department, Saudi Arabia , Ahmed Zaghloul, Mohamed Salman Bin Abdulaziz University - College of Pharmacy - Pharmacognosy Department, Saudi Arabia , Ahmed Zaghloul, Mohamed Mansoura University - College of Pharmacy - Pharmacognosy Department, Egypt
Abstract :
The current research was designed to evaluate the phytochemical contents, cytotoxic and antimicrobial activity of Emex spinosa extracts. The different plant extracts and Aloe-emodin glucoside were screened using the colorimetric MTT method (3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl- tetrazolium bromide) assay to test their in vitro cytotoxic activity against HepG2, MCF-7, Caco-2 and HCT. The clinically used anticancer drug doxorubicin was used as standard for comparative purposes. Anthraquinones (Aloe-emodin-O-glucoside, Emodin and nataloin (1, 2, 8-trihydroxy, 6-methyl, 10-anthrone-C-glucoside) together with β-sistosterol and β-sitisterol-O-β-D-glucoside were isolated from Emex spinosa. Aloe-emodin glucoside together with four fractions from this plant were evaluated for their anticancer and antimicrobial activities. Aloe-emodin glucoside showed anticancer activity against HCT, HepG-2, MCF-7 and Caco-2 cell lines. The total ethanol extract of E. spinosa and diethyl ether, chloroform, ethyl acetate and butanol fractions shown antibacterial activity against Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilis.
Keywords :
The current research was designed to evaluate the phytochemical contents , cytotoxic and antimicrobialactivity of Emex spinosa extracts. The different plant extracts and Aloe , emodin glucoside were screened using thecolorimetric MTT method (3 , (4 , 5 , dimethylthiazo , 2 , yl) , 2 , 5 , diphenyl , tetrazolium bromide) assay to test their in vitrocytotoxic activity against HepG2 , MCF , 7 , Caco , 2 and HCT. The clinically used anticancer drug doxorubicin was used asstandard for comparative purposes. Anthraquinones (Aloe , emodin , O , glucoside , Emodin and nataloin (1 , 2 , 8 , trihydroxy , 6 , methyl , 10 , anthrone , C , glucoside) together with β , sistosterol and β , sitisterol , O , β , D , glucoside were isolated fromEmex spinosa. Aloe , emodin glucoside together with four fractions from this plant were evaluated for their anticancer andantimicrobial activities. Aloe , emodin glucoside showed anticancer activity against HCT , HepG , 2 , MCF , 7 and Caco , 2cell lines. The total ethanol extract of E. spinosa and diethyl ether , chloroform , ethyl acetate and butanol fractions shownantibacterial activity against Staphylococcus aureus , Streptococcus pyogenes and Bacillus subtilis.
Journal title :
Pakistan Journal Of Pharmaceutical Sciences
Journal title :
Pakistan Journal Of Pharmaceutical Sciences
