Author/Authors :
Khan, Zulfiqar Ali Government College University - Department of Chemistry, Pakistan , Naqvi, Ali Raza Government College University - Department of Chemistry, Pakistan , Mukhtar, Ammara Government College University - Department of Chemistry, Pakistan , Hussain, Zaib University of the Punjab - Institute of Chemistry, Pakistan , Shahzad, Sohail Anjum COMSATS institute of information technology - Department of Chemistry, Pakistan , Mansha, Asim Government College University - Department of Chemistry, Pakistan , Ahmad, Matloob Government College University - Department of Chemistry, Pakistan , Zahoor, Ameer Fawad Government College University - Department of Chemistry, Pakistan , Bukhari, Iftikhar Hussain Government College University - Department of Chemistry, Pakistan , Janjua, Muhammad Ramazan Saeed Ashraf University of Sargodha - Department of Chemistry, Pakistan , Mahmood, Nasir University of Health Sciences - Department of Allied Sciences and Chemical Pathology, Pakistan , Yar, Muhammad COMSATS Institute of Information Technology - Interdisciplinary Research Center in Biomedical Materials, Pakistan
Abstract :
Antioxidant and antibacterial potential of different solvent extracts of locally grown Hibiscus rosa-sinensis Linn was evaluated. The antioxidant activity was assessed by estimation of total flavonoids contents, total phenolic contents, DPPH free radical scavenging activity and percentage inhibition of linoleic acid oxidation capacity. Agar disc diffusion method was used to assess antibacterial potential of crude extract of H. rosa-sinensis. The yield of the crude extracts (23.21±3.67 and 18.36±2.98% in 80% methanol and ethanol solvents was calculated, respectively. Methanol and ethanol extract of H. rosa-sinensis showed total phenolics 61.45±3.23 and 59.31±4.31 mg/100g as gallic acid equivalent,total flavonoids 53.28±1.93 and 32.25±1.21 mg/100g as catechine equivalent, DPPH free radical scavenging activity 75.46±4.67 and 64.98±2.11% and inhibition of linoleic acid oxidation potential 75.8±3.22 and 61.6±2.01% respectively, was measured. Antibacterial study against three human pathogens such as staphlococus sp. Bacillus sp. and Escherichia coli showed growth inhibitory effect in the range of 12.75±1.17 to 16.75±2.10 mm. These results showed H. rosasinensis indigenous to Kallar Kahar and its allied areas bear promising medicinal values and could be used for developing herbal medicines to target oxidative stress and infectious diseases.
Keywords :
H. rosa , antibacterial activity , Kallar Kahar , antioxidant , diabetes
