Author/Authors :
Ismail, Noor Aisyah Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Shamsahal Din, Nor Syafawati Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Mamat, Siti Syariah Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Zabidi, Zalina Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Zainulddin, Wan Noraziemah Wan Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Kamisan, Farah Hidayah Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Yahya, Farhana Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Mohtarrudin, Norhafizah Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Pathology, Malaysia , Mohd Desa, Mohd Nasir Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Sciences, Malaysia , Zakaria, Zainul Amiruddin Universiti Putra Malaysia - Faculty of Medicine and Health Science - Department of Biomedical Sciences, Malaysia , Zakaria, Zainul Amiruddin Universiti Teknologi MARA - Pharmacogenomics Center, Faculty of Pharmacy, Puncak Alam Campus, Malaysia
Abstract :
The present study aimed to determine the hepatoprotective activity of Dicranopteris linearis L. (family Gleicheniaceae) leaf aqueous extract (DLAE) using two models of liver injury in rats. Rats were divided into ten groups (n=6) and received dH2O (negative control), 200 mg/kg silymarin (positive control) or DLAE (50, 250 and 500 mg/kg) orally once daily for 7 consecutive days and on the 8th day subjected to the hepatotoxic induction either using carbon tetrachloride (CCl4) or paracetamol (PCM). The bloods and livers were collected and subjected to biochemical and microscopical analysis. From the data obtained, only the highest dose of DLAE significantly (p 0.05) reduced the ALP, ALT and AST levels in CCl4-and PCM-induced hepatotoxic rats while the other doses caused significant (p 0.05) reduction only in the levels of ALT and AST. The histological results obtained were in line with the biochemical analysis wherein reduction in the CCl4- and PCM-induced tissue formation of necrosis, steatosis and inflammation occurred in a dose-dependent manner. In conclusion, the DLAE possesses hepatoprotective activity, which could be attributed to its free radicals scavenging and antioxidant activities, and high flavonoids content. Thus, in-depth studies regarding the hepatoprotective activity of DLAE are warranted.
Keywords :
Dicranopteris linearis , Gleicheniaceae , in vivo , hepatoprotective activity , aqueous extract , leaves
