Author/Authors :
Sajjad, Naheed University of Karachi - Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), Pakistan , Sajjad, Naheed Sardar Bahadur Khan Women’s University (SBKWU), Pakistan , Haque, Sayedul University of Karachi - Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), Pakistan , Haque, Sayedul Quaid-e-Azam University - Human Genetics Program, Department of Animal Sciences, Pakistan , Burney, Intesar National Research Institute for Fertility Care (NRIFC), Pakistan , Shahid, Muhammad University of Karachi - Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), Pakistan , Zehra, Sitwat University of Karachi - Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), Pakistan , Azhar, Abid University of Karachi - Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), Pakistan
Abstract :
The identification of chromosomes for routine cytogenetic analysis is based on quality of metaphases and good banding pattern. Fresh slides of human lymphocytes have been shown to produce good bands for the identification of chromosomes morphology. G-bands by Trypsin using Giemsa (GTG) banding of aged slides is generally considered hard to get desired band pattern of chromosomes persistently. The current study is focused on GTG banding of aged slides. A total of 340 subjects including 290 primary infertile and 50 fertile were selected. The blood samples were drawn aseptically for cytogenetic analysis. Lymphocytes were cultured and GTG banding was done on 1440 glass slides. Giemsa trypsin banding of aged slides were done by adjusting average trypsin time for each month according to the slide age and metaphase concentration. Correlation analyses showed a significant and positive correlation between slide ageing and trypsin pre-treatment time. The results of this study suggest that, the fresh and aged human lymphocyte metaphases are equally usable for GTG banding.
Keywords :
Cell culturing , chromosomes , cytogenetic , GTG banding , karyotyping
