Author/Authors :
nikbin, v.s. department of bacteriology, pasteur institute of iran, ايران , alikhani, m.y. isfahan university of medical sciences, ايران , shojaei, h. hamadan university of medical sciences, ايران , mousavi, s.f. department of bacteriology, pasteur institute of iran, ايران , salmanzadeh-ahrabi, s. alzahra university, تهران, ايران , aslani, m.m. department of bacteriology, pasteur institute of iran, ايران
Abstract :
Aim: We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping. Background: Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children. Patients and methods: A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping. Results: Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the six gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters. Conclusion: According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa. © 2012 RIGLD, Research Institute for Gastroenterology and Liver Diseases.
Keywords :
Diarrhea , EPEC , Ribotyping , Serotyping
