NANOSPIDER TECHNOLOGY FOR CONTROLLING OF PSEUDOMONASCICHORII AND DICKEYA DADANTII BY ELECTROSPUN NANOFIBERSOF NYLON-6/CHITOSAN BLENDS

This is the first report on the use of electrospun nanofibers whichcould be of considerable interest to the development of new antibacterialcompounds against two species of bacteria: Pseudomonas cichorii causingbacterial leaf spot (bacterial midrib rot) and Dickeya dadantii (Erwiniachrysanthemi) causing bacterial bligh. Electrospun nylon-6/chitosan (nylon-6/Ch)nanofibers were obtained using formic acid as a single solvent. Surfacemodification of electrospun nylon-6/chitosan nanofibers was performed by soakingthe mat in an aqueous solution of glycidyltrimethylammonium chloride (GTMAC)at room temperature overnight to give nylon-6/N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride (nylon-6/HTCC). The morphological,structural and thermal properties of the nylon-6/chitosan nanofibers were studiedby field-emission scanning electron microscopy (FE-SEM), X-ray diffraction(XRD), Fourier transform-infrared (FT-IR) spectroscopy, and thermogravimetricanalysis (TGA). Biological screening demonstrated that Nylon-6/HTCC matexhibited high potential antibacterial activity on protein synthesis of bacteriaPseudomonas cichorii and Dickeya dadantii. Bacteria examined using SEM weretotally deformed and exhibited symptoms of severe destruction.