Author/Authors :
olamitibo, daramola james federal university of agriculture - department of animal physiology, Nigeria , dayo, onanuga oluwakemi federal university of agriculture - department of animal physiology, Nigeria , oladimeji, abioja monsuru federal university of agriculture - department of animal physiology, Nigeria , mathew, adeleke federal university of agriculture - animal breeding and genetics department, Nigeria , olajide, olowofeso federal university of agriculture - animal breeding and genetics department, Nigeria , emmanuel, oke oyegunle federal university of agriculture - department of animal physiology, Nigeria , oluwafemi, adekunle ezekiel federal university of agriculture - department of animal physiology, Nigeria , amidu, sorongbe taiwo federal university of agriculture - department of animal physiology, Nigeria , ayobami, iyanda olayinka federal university of agriculture - department of animal physiology, Nigeria
Abstract :
Effects of avocado seed extract (ASE) in different tris-extenders on sperm and seminal oxidative stress indices of vitrified spermatozoa of West African Dwarf (WAD) goats during vitrification were assessed. Semen samples were diluted with varying levels (0, 5, 10, 15 and 20 mL/100 mL of diluent) of ASE in tris citric acid (TCA), tris sodium phosphate (TSP) and tris sodium citrate (TSC) extenders while a tris-extender without citric acid, sodium phosphate and sodium citrate served as control. Diluted semen samples were vitrified and preserved. The results revealed higher (P 0.05) progressive sperm motility in TCA extenders at 10%, 15% and 20%, and TSP extender at 20% ASE. Acrosome integrities were higher (P 0.05) in the different tris-extenders at 15% and 20% ASE. TCA, TSP and TSC extenders had higher (P 0.05) membrane integrities at 10%, 15% and 20% ASE. TSP extenders had higher (P 0.05) live sperm at 20% ASE. TCA extenders had higher acrosome reaction while TSP at 20% ASE and TSC at 15% and 20% of ASE had higher sperm capacitation. TCA extender at 20% ASE had reduced malondialdehyde (MDA) concentrations while higher acrosin activities were observed at 20% ASE in TCA extender (P 0.05). The findings indicated that 20% ASE in the tris-extenders improved sperm and oxidative stress indices and the tris-extenders were ranked best in this order: TCA TSP TSC.
Keywords :
antioxidant , freezing , bucks , oxidative stress , semen extenders , sperm quality
