Bovine oocyte maturation collected from abattoir and in vitro fertilization

This study was conducted at biotechnology center in Baghdad. Eighty (80) of bovine ovaries from Al-Shualah abattoir in Baghdad after slaughter have been collected. The total number of oocytes were obtained from ovaries by aspiration of follicles reached (71). The follicle selection method according to size from 3-7 mm. This oocytes classified into two groups according to the cumulus cells surrounded the oocyte, partially or completely. The first group included (37) oocytes completely surrounded by cumulus cells, and the second group (34) oocytes without cumulus cells. All oocytes subjected to incubation in TCM-199 medium at 38 C˚ in CO2 incubator about 24 hrs., the results indicates that (24) 64.88% of the oocytes in the first group and (16) 47.05% of the second group showed maturation. All matured oocyte were fertilized by frozen semen which had been brought from artificial insemination center (Abu Ghraib) as straws. The result appeared (13) 54.16% fertilized oocytes in first group while the number of fertilized oocyte in second group reached (6) 46.15%, when incubation had been continued for further development, the cumulus oocyte complex give (6) 37.50% embryos in two cells to four cells (Cleavage stages). But the second group give only (2) 33.33% embryos. No significant differences in maturation between oocyte with cumulus (WC) and without cumulus (WOC) among oocyte (p 0.05).