Pathological study of therapeutic Effect of Crude Extract of Euphorbia granulata F.in experimentally implanted Murine Mammary Adenocarcinoma

This study was designed to evaluate the cytotoxic effect of 70% ethanolic crude extract of Euphorbia granulata F. on normal and cancer cells. The acute toxicity of 70% ethanolic extract of the plant on normal mice has been studied. LD50 was determined by probit study (1486 mg/ kg B.W.) to calculate the therapeutic dose which was 30 mg/kg B.W S/C injection. Therapeutic effect of ethanolic extract of E. granulata was studied on tumorbearing female mice after subcutaneous administration of 30 mg/kg B.W for 30 days (group II), while tumor-bearing female mice (group I) have been injected with distilled water only and served as control. The results were showed a significant reduction in tumor volume, relative tumor volume and inhibition of tumor growth rate in treated mice (group II) compared with tumor-bearing female mice of non-treated group (group I). Histopathological sections were showed large areas of necrosis, mononuclear cell infiltration and granulation tissue formation surrounding the tumor cells in tumor-bearing female mice (treated group II), hyperplasia of white pulp of the spleen with amyloid deposition and proliferation of megakaryocyte. Mononuclear cell infiltrations in liver, kidney, intestine and brain (group II) compared with non treated tumor bearing female mice (group I). The tumor growth was showed acinar like structures with milk like secretion, pleomorphic hyperchromatic tumor cells, and calcification of necrotic area. Liver showed small aggregation of tumor cells within liver parenchyma with extensive areas of coagulative necrosis and apoptosis. kidney undergo hydropic degeneration with dilation of urinary space. Brain showed perivascular and perineuronal edema with congestion, while spleen showed mild hyperplasia and proliferation of megakaryocytes. Tumor associated macrophages were infiltrated in liver and lung of control group.