Abstract :
The present study was carried out to observe histopathological changes in tumor mass of transplanted mammary adenocarcinoma tumor cells, spleen, liver and kidney of female albino mice successfully showed growth of tumor cells (group I) following subcutaneous transplantation with mammary gland adenocarcinoma cell line, comparison to the other group of female albino mice not exhibited any tumor growth post transplantation (group II). Group (III) of female albino mice were injected subcutaneously with phosphate buffer saline and served as normal healthy control group. The cell line which had been used in the study was mice mammary gland adenocarcinoma (Ahmad-Majed 2003) adapted for in vivo transplantation supplied from Iraqi Center for Cancer and Medical Genetic Researches (ICCMGR).Thirty female albino mice were used in this study. Twenty female albino mice transplanted subcutaneously with mammary gland adenocarcinoma cell line. Six female albino mice showed successful growth of transplanted tumor cells as a tumor mass after 10 days post transplantation, tumor mass showed increase in their size at the end of experiment(40days) post transplantation and considered as a (group I). while fourteen female albino mice not exhibited any growth of tumor cells after 10 days post transplantation until the end of experiment (40days) post transplantation and considered as (group II).while10 female albino mice were injected subcutaneously with phosphate buffer saline and served as normal healthy control group (group III ) . At the end of the experiment (40) days post transplanted, animals of all groups were scarified and tissue of growth tumor mass in group I, spleen, liver and kidney of all groups were taken for histopathological examination. Histopathological results of tumor mass of group (I), revealed extensive tumor masses consisted of aciner like structure, trabecular and island of tumor cells, the tumor cells are hyperchromatic, pleomorphic, increase the nuclear–cytoplasmic ratio with highly blood supply, the tumor masses also showed extensive area of coagulation necrosis. Also histopathological sections showed depletion of white pulp region of spleen, vacuolar degeneration in hepatocytes and atrophy of glomarular tuft with vacuolar degeneration of renal tubules. While histopathological sections of group (II) showed extensive hyperplasia of white pulp in the periarterial sheath (T and B cell region) of spleen, mononuclear inflammatory cells infiltration (lymphocyte and macrophage) showed around central vein, portal area of liver. In addition, the kidney revealed infiltration of mononuclear inflammatory cells in interstitial tissue and around B.V. Microscopic examination of (group III) showed no histopathological changes in spleen, liver and kidney compared with (group I (and (group II).
